Coding

Part:BBa_K4202010:Design

Designed by: Hongying LIN   Group: iGEM22_ZJU-China   (2022-09-30)


Amorphous Calcium CarbonateBinding Protein(ACCBP)(without the 16 amino acids at N terminus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 45
    Illegal AgeI site found at 308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 514


Design Notes

BBa_K4202010 is a part of ACCBP without signal sequence.Besides,to test the effect of N-terminal amino acid sequence on protein folding，we delete 16 amino acids at N terminus.


Source

The sequence of this part was originally retrieved from GenBank DQ473430.1. The codons of this sequence was optimized by Genscipt.

References